Expression of ATP1B3 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ATP1B3 (extracellular) Antibody (#ANP-013), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the in rat hippocampal dentate gyrus region, revealed immunoreactivity (green) in neurons of the hilus (H, arrows) and the granule layer (G). B. Pre-incubation of the antibody with ATP1B3 (extracellular) Blocking Peptide (BLP-NP013), suppressed staining. Cell nuclei are stained with DAPI (blue).

Peptide (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase (Accession P54709). Extracellular, C-terminus.

Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4): - 1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200). 3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013)